ILC2s were sorted from the MLN of IL-25-treated mice
antibody
anti-Gfi1 (a custom rabbit polyclonal antibody raised against N- and C-terminal peptides)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
~3 x 10^7 cells were harvested, crosslinked with formaldehyde, sonicated, IPed with appropriate antibodies and reverse-crosslinked to obtain 300-500 bp DNA fragments. Standard Illumina kit was used for library construction.