Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gfi1

Cell type

Cell type Class
Blood
Cell type
ILC2
NA
NA

Attributes by original data submitter

Sample

source_name
Innate type-2 lymphocytes
strain
C57BL/6
cell type/growth condition
ILC2s were sorted from the MLN of IL-25-treated mice
antibody
anti-Gfi1 (a custom rabbit polyclonal antibody raised against N- and C-terminal peptides)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
~3 x 10^7 cells were harvested, crosslinked with formaldehyde, sonicated, IPed with appropriate antibodies and reverse-crosslinked to obtain 300-500 bp DNA fragments. Standard Illumina kit was used for library construction.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
49361770
Reads aligned (%)
98.0
Duplicates removed (%)
22.3
Number of peaks
3031 (qval < 1E-05)

mm9

Number of total reads
49361770
Reads aligned (%)
97.7
Duplicates removed (%)
22.3
Number of peaks
3024 (qval < 1E-05)

Base call quality data from DBCLS SRA